The nerf is notable and potentially extremely annoying depending on how and when it kicks in. Annoying enough to cheat around it? Possibly, but I hope not. I'd only just managed to find bees enjoyable thanks to Extra Bees making the genetic minigame massively less annoying to work with and now something threatens to wreck it again, bleh! So long as you can get guaranteed production from a perfectly bred bee it's fine by me, otherwise it's time for cheating. I do, however, appreciate that trying to push a bee past 100% should probably kill it - but in that case the bee's performance should be less obtuse and easier to track.
A 16 multiplier on the alveary is pretty darn forgiving (4 frames). Assuming you breed in ideal traits you are looking at 100% on a lot of products, and a very good rate on even the rarest. Who this is really going to hurt is the people who do not engage with bees enough to breed in ideal traits and just brute force it with more frames. Also a 10 mutation chance multiplier will be enough to get a lot of the more basic mutations up to garunteed, and the rare ones up to a very respectable chance. Combined with the buff to swarmer lives that I saw a while back it will mean that you need to put just a bit more effort into your system, but you will still be able to rely on bees. Maybe that is optimistic thinking though, since I am just getting started on a game where I am trying not to mine a single ore and will be relying on bees a lot
Anyway, on our server at the moment we're finding on bees bred for optimal traits (maximum production, pollination, life, etc.) six impregnated frames is always enough for 100% production even on bees with very rare specialties. This nerf may prove very irritating for those of us trying to obsolete the quarry!
This is correct, 6 frames will always get a fastest bee up to 100% even on a 1% base.