Which bee has the Shortest lifespan trait

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You should check the wiki next time, there is a list of bee species by lifespan.

The easiest one to get would be rocky bees.
 
are you saying you bred them and they didn't show the "shortest" gene or that you just weren't able to get a serum from them?
 
Serums is all by chance . Some easier then others . The chance is affected by which bee you use sometimes you just need to use multiple of the same bee to get it. You can smelt the serum if its not what you want and reuse the vial
 
Common bees are a better bet. Rocky lack fertility, so with those you're more likely to simply run out of bees.
 
Common bees are a better bet. Rocky lack fertility, so with those you're more likely to simply run out of bees.


The other traits of the bee are irrelevant when trying for a serum though. I'm actually tempted to agree with this, however, as Commons are a lot easier to get (At least for me, and mostly, pre-quarry stages of the game)